After getting a sizable portion of ablated embryos fixed in ethanol at the necessary stages (st. 18 and st. 30), in situ hybridization is necessary to determine the gene expression patterns in the embryos. This procedure works by linearizing a large amount of plasmid DNA grown from E. coli cultures. E. coli is used to gather a large amount of DNA for use because they reproduce quickly. After linearizing the DNA, transcription is done in vitro to create antisense RNA that will bind to the location of the RNA for the gene of interest. This RNA is then labeled with fluorescent or colored probes so that the signal can be observed. The genes of interest used in this experiment are XCG-1, Otx2, En2, and Krox20. These are various neural marker genes that are expressed early and later in development. A control group of embryos also undergoes the hybridization procedure as a control to show where normal expression occurs in these embryo stages.
When looking at the value of anything, we can take two approaches. The first approach: straight forward reasoning. If a bottle of water costs slightly over two cents to make, then its value should be right around that amount. The second: the free market approach. If people are willing to pay $4.25 for a bottle of water at the movie theater, then the bottle of water should be valued at $4.25. [Read more…]
As I am continuing with my research, I have found that so many historical events contributed to the Jamaicans’ view of Haile Selassie as a “god”. For example, a seemingly unconnected event, the foundation of the UNIA (Universal Negro Improvement Association) by Marcus Garvey, a black rights activist in 1914, indirectly influenced this phenomenon as well. Garvey’s objectives were to claim or reclaim a black identity of pride, freedom, and autonomy through education, organization, and centralization. For example, one of his mottos was “One God, One Aim, One Destiny”. A direct quote from Garvey, before he traveled to England, was “Look to Africa, when a black king shall be crowned, for the day of deliverance is at hand”. After receiving acclaim in the United States, Marcus Garvey also found a following in Jamaica after he organized strikes in the ports to seek better rights for black workers. These Jamaican followers called themselves Garveyites, and were the forefathers of the Rastafari movement. Garvey’s quote was then used as a prophesy by Rastafari.
Welcome to my last Freshman Monroe post! For the last few weeks, I have been looking to see if the presence of Médecins Sans Frontières (MSF) in conflict zones has an effect on the formation of lasting peace. After reading several books and articles (my findings from each can be found here and here), I decided that I would be able to produce more thorough results if I made the project a case study.