Blog post #2 – Erasure, Law, Resistance

#2

This will be a common burial-place where all shall meet on terms of common fellowship and brotherhood. Every dear relation in life, severed by death, shall be found restored again in these grounds— husband and wife, parent and child, brother and sister, shall be reunited here. Friend shall meet friend here; and enemies, too, shall meet, there enmities all forgotten. Yonder city, where, as every where in life, the harmonies of society are apt to be broken by petty feuds, by ungentle rivalries, by disturbing jealousies, by party animosities, by religious dissensions, shall, one after another, as death singles them out, send up her multitudinous population to these grounds, and here they shall take their respective places, in amiable proximity to each other, peaceful, harmonious, undisturbed and undisturbing, the same shadows deepening on them, the same sun-light over them, resting in the same hope…

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Ideology of the Cemetery, Ideology of Archaeology, Ideology of Resistance

It is presumably time that I make my first blog post. Here, I hope to detail three things:

  1. My renewed understanding of struggles for liberation and sovereignty thanks to the educational work of Sierra’s SPROG
  2. Notices through preliminary research of the history of disrupted human remains for marginalized communities
  3. Randall H. McGuire’s “Ideology of the Cemetery” as it relates to the previous two items

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Working with Dansyl Chloride-Labeled Glutathione, Blog Post #3: Wrapping It Up

Hey, everyone! It’s been a good while since I wrote my second blog post, and it’s actually been a couple of weeks since I finished working in Dr. Landino’s lab for the summer. During the interim I’ve been gathering and organizing data from our experiments up to the eighth week of the summer (a period extending from May 31 to July 20): this includes creating data tables detailing our experimental concentrations and fluorescence intensity results as well as organizing our photographed images and notes into a long slideshow. At present I’m working on creating a final, large data table that displays the general results and reactivity trends we observed over the summer; the table will make developing a display for the Freshman Monroe Grant project exhibition a much simpler task.

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Working with Dansyl Chloride-Labeled Glutathione, Blog Post #2: Introducing the Polyacrylamide Gel

Hey, everyone! It’s been quite a while since my last post, so it’s high time for me to give you an update as to what has been going on in the Landino lab. Well, once we finished trying to re-oxidize reduced dansyl chloride-labeled glutathione (DGSH) and reduce oxidized single dansyl-labeled glutathione (DGSSG) and double dansyl-labeled glutathione (DGSSGD), we began working with polyacrylamide gels. These gels are especially important in our lab, as through them we can separate the proteins and materials we regularly use in our reactions. Once separated, any fluorescent labels or labeled material (often protein) in the gel can be visualized (usually as bands or smears) with a specialized camera-like apparatus we have in the lab. While extremely useful, the gels take some time to make, and it is always worrisome when we have to deal with acrylamide. (Acrylamide itself is a neurotoxin; polyacrylamide, fortunately, is not, so the hardened/polymerized gels are essentially harmless.) However, by now we have made and run reactions in these gels so many times that it is like second nature, and I frankly enjoy doing it.

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