CRISPR and Ethics, Update 2: Planning the Review

For those just tuning in, my project this summer is to write a literature review concerning the ethical implications of CRISPR-cas9 gene-editing technology.   For the first part of my project I focused on understanding the mechanism of CRISPR itself, in order to have a strong knowledge base with which to approach the ethical aspects.  In brief, CRISPR is based on a naturally-occurring anti-viral system which exists in bacteria.  This system can be manipulated in order to delete or insert genes into the target strand.  In my recent work I began delve into what this means CRISPR is capable of, and I have had to redirect the final goal of my project.

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C. elegans Research: Update 3/Final Post!

Although I finished my work on campus a few months ago, I had a lot more analysis to do once I returned home! In order to finish my project and come up with some conclusions, I used photoshop to analyze the images I captured with the epifluorescent microscope. To study these photographs, I viewed the different layers in each image; this allowed me to see different cell parts, such as the DNA and tubulin, individually and as part of the entire picture. Because I completed three different types of immunopreps: DNA/tubulin staining, DNA/actin staining, and DNA/ERM-1 staining (see post #2 for an explanation), I had to analyze each type of prep separately to understand the dynamics of tubulin, actin, and ERM-1 in the mutant C. elegans strain that I was working with in lab. Studying the images I obtained from each prep helped me to understand what went wrong with each of the three proteins (listed above) during the process of spermatogenesis.

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C. Elegans Research: Update #2

(Originally written 6/11/18)

            I have finished the wet lab portion of my project, and I learned so much while working on campus!

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C. Elegans Research: Update #1

(originally written 5/29/18)

            In my project, I will be studying spe-26 mutant C. elegans, in order to gain insights into the function of the SPE-26 protein. If this sounds like jargon to you, don’t worry; I’ll explain!

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